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tissue culture u937 cells  (ATCC)


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    ATCC tissue culture u937 cells
    Tissue Culture U937 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+culture+u937+cells/U-937/10__7554_slash_elife__91157__3-147-3-10
    Average 99 stars, based on 6884 article reviews
    tissue culture u937 cells - by Bioz Stars, 2026-10
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    other:

    Article Title: Targeting host deoxycytidine kinase mitigates Staphylococcus aureus abscess formation
    Article Snippet: Cell lines and tissue culture U937 cells were obtained from American Type Culture Collection (ATCC) and grown in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco) supplemented with 10% heat- inactivated fetal bovine serum (hi- FBS) according to the manufacturer’s instructions.



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    tissue culture u937 cells  (ATCC)
    99
    ATCC tissue culture u937 cells
    Tissue Culture U937 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+culture+u937+cells/U-937/10__7554_slash_elife__91157__3-147-3-10
    Average 99 stars, based on 1 article reviews
    tissue culture u937 cells - by Bioz Stars, 2026-10
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    tissue culture cell growth conditions human derived u937 macrophages  (ATCC)
    99
    ATCC tissue culture cell growth conditions human derived u937 macrophages
    Fig. 6. LLO is dispensable for cell-to-cell spread between human-derived <t>macrophages</t> and human epithelial cells. Human-derived <t>U937</t> macrophages were infected in the absence of IPTG with pre-induced iLLO, DPLCs iLLO or DPI-PLC iLLO bacteria at a moi of 50:1. At 6 h post infection, infected U937 macrophages were added to monolayers of Hep2 epithelial cells at a moi of 2:3 (primary to secondary host cell ratio) in the presence of gentamicin and in the absence of IPTG (secondary infection). Monolayers were overlaid 3 h post secondary infection with an agarose medium mixture containing gentamicin without IPTG. Plaques within the monolayers were visualized 5–6 days post secondary infection. A. Heterologous plaquing assay with iLLO bacteria. A total of 4 ¥ 105 infected U937 (representing a total of 1–2 ¥ 106 bacteria) were used to infect a monolayer of Hep2 cells. Arrows point to representative plaques. Each plaque is the result of a successful heterologous cell-to-cell spread event followed by subsequent cell-to-cell spreading and Hep2 cell lysis. B. Heterologous plaquing assay with DPLCs iLLO bacteria. C. Heterologous plaquing assay with DPI-PLC iLLO bacteria. D. Monolayers of Hep2 cells were left uninfected. E. A total of 1.25 ¥ 108 wild-type bacteria were added directly to Hep2 cells in the presence of gentamicin. F. U937 lysate (representing a total of 1–2 ¥ 106 iLLO bacteria) were added directly to Hep2 cells in the presence of gentamicin.
    Tissue Culture Cell Growth Conditions Human Derived U937 Macrophages, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+culture+u937+cells/U-937/pm17222191-260-0-8
    Average 99 stars, based on 1 article reviews
    tissue culture cell growth conditions human derived u937 macrophages - by Bioz Stars, 2026-10
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    Fig. 6. LLO is dispensable for cell-to-cell spread between human-derived macrophages and human epithelial cells. Human-derived U937 macrophages were infected in the absence of IPTG with pre-induced iLLO, DPLCs iLLO or DPI-PLC iLLO bacteria at a moi of 50:1. At 6 h post infection, infected U937 macrophages were added to monolayers of Hep2 epithelial cells at a moi of 2:3 (primary to secondary host cell ratio) in the presence of gentamicin and in the absence of IPTG (secondary infection). Monolayers were overlaid 3 h post secondary infection with an agarose medium mixture containing gentamicin without IPTG. Plaques within the monolayers were visualized 5–6 days post secondary infection. A. Heterologous plaquing assay with iLLO bacteria. A total of 4 ¥ 105 infected U937 (representing a total of 1–2 ¥ 106 bacteria) were used to infect a monolayer of Hep2 cells. Arrows point to representative plaques. Each plaque is the result of a successful heterologous cell-to-cell spread event followed by subsequent cell-to-cell spreading and Hep2 cell lysis. B. Heterologous plaquing assay with DPLCs iLLO bacteria. C. Heterologous plaquing assay with DPI-PLC iLLO bacteria. D. Monolayers of Hep2 cells were left uninfected. E. A total of 1.25 ¥ 108 wild-type bacteria were added directly to Hep2 cells in the presence of gentamicin. F. U937 lysate (representing a total of 1–2 ¥ 106 iLLO bacteria) were added directly to Hep2 cells in the presence of gentamicin.

    Journal: Cellular microbiology

    Article Title: Differential function of Listeria monocytogenes listeriolysin O and phospholipases C in vacuolar dissolution following cell-to-cell spread.

    doi: 10.1111/j.1462-5822.2006.00780.x

    Figure Lengend Snippet: Fig. 6. LLO is dispensable for cell-to-cell spread between human-derived macrophages and human epithelial cells. Human-derived U937 macrophages were infected in the absence of IPTG with pre-induced iLLO, DPLCs iLLO or DPI-PLC iLLO bacteria at a moi of 50:1. At 6 h post infection, infected U937 macrophages were added to monolayers of Hep2 epithelial cells at a moi of 2:3 (primary to secondary host cell ratio) in the presence of gentamicin and in the absence of IPTG (secondary infection). Monolayers were overlaid 3 h post secondary infection with an agarose medium mixture containing gentamicin without IPTG. Plaques within the monolayers were visualized 5–6 days post secondary infection. A. Heterologous plaquing assay with iLLO bacteria. A total of 4 ¥ 105 infected U937 (representing a total of 1–2 ¥ 106 bacteria) were used to infect a monolayer of Hep2 cells. Arrows point to representative plaques. Each plaque is the result of a successful heterologous cell-to-cell spread event followed by subsequent cell-to-cell spreading and Hep2 cell lysis. B. Heterologous plaquing assay with DPLCs iLLO bacteria. C. Heterologous plaquing assay with DPI-PLC iLLO bacteria. D. Monolayers of Hep2 cells were left uninfected. E. A total of 1.25 ¥ 108 wild-type bacteria were added directly to Hep2 cells in the presence of gentamicin. F. U937 lysate (representing a total of 1–2 ¥ 106 iLLO bacteria) were added directly to Hep2 cells in the presence of gentamicin.

    Article Snippet: Tissue culture cell growth conditions Human-derived U937 macrophages (ATCC CRL-1593.2) and human Hep2 epithelial cells (ATCC CCL-23) were cultured in RPMI medium (Mediatech, Herndon, VA) supplemented with 10% fetal bovine serum (FBS; HyClone, Logan, UT), 2 mM glutamine, 1 mM sodium pyruvate, 100 mg ml-1 penicillin and streptomycin (P/S) and 55 mM b-mercaptoethanol (b-ME) (RPMI10).

    Techniques: Derivative Assay, Infection, Bacteria, Lysis